Electrochemical biosensor for glycine detection in biological fluids

We present herein the very first amperometric biosensor for quantitative determination of glycine in diverse biological fluids. The is based on a novel quinoprotein that catalyzes oxidation with high specificity. This process coupled to redox conversion Prussian blue presence hydrogen peroxide originating from enzymatic reaction. optimized tailoring design consists effective encapsulation chitosan matrix posterior addition an outer Nafion layer, which here demonstrated suppress interference. particularly important case ascorbic acid, known influence behavior blue. analytical performance demonstrates fast response time (<7 s), acceptable reversibility, reproducibility, and stability (<6% variation) as well wide linear range (25–500 ?M) covers healthy (and even most unhealthy) physiological levels blood/serum, urine sweat. A total 6 real samples patients animals were analyzed: two serum, sweat samples. results validated via commercially available fluorescence kit, displaying discrepancy less than 9% all unique features effortless preparation new position it at forefront current technologies towards decentralized clinical applications sport monitoring.